Journal: Molecular cell
Article Title: Homology requirements and competition between gene conversion and break-induced replication during double-strand break repair
doi: 10.1016/j.molcel.2016.12.003
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Anti-Rad53 antibody Abcam ab166859 Chemicals, Peptides, & Recombinant Proteins Taq DNA Polymerase Promega M300F Fail Safe 2X PCR Mix E - Buffer E Epicenter FSP995E AseI New England Biolabs R0526M NEB Buffer 3.1 New England Biolabs B7203S Bsp1286I New England Biolabs R0120L ClaI New England Biolabs R0197S 10X Cut Smart Buffer New England Biolabs B7204S Easy Tides Deoxyadenosine 5′ triphosphate ( 32 P) Perkin Elmer BLU512H250HC GeneScreen Plus Nylon Hybridization Transfer Membrane Perkin Elmer NEF988001PK Agarose Low EEO (EP) Electrophoresis Grade US Biological A1016 Critical Commercial Assays AccuPrep Plasmid Mini Extraction Kit Bioneer K3030 Gene Jet PCR Purification Kit Thermo Scientific K0702 MasterPure Yeast DNA Purification Kit Epicentre MPY80200 DECA primer II - Random Primed DNA Labeling Kit Ambion AM1456 Experimental Models: Organisms/Strains See Table S1 for a list of yeast strains used in this study Sequence Based Reagents See Table S2 for a list of all oligonucleotides used in this study IDT (Integrated DNA Technologies) Software and Algorithms Bio-Rad Quantity One Software Version 4.5.2 Bio-Rad www.bio-rad.com Image Quant TL Version 5.2 GE Healthcare www.gelifesciences.com Lasergene SeqBuilder DNAStar www.dnastar.com/t-seqbuilder.aspx Rotor Gene Q software Version 2.2.3 Qiagen www.qiagen.com Equipment Bio-Rad Mini PROTEAN Tetra System Bio-Rad #165-8004 Bio-Rad Mini Trans-Blot Cell Bi0-Rad #1703930 Open in a separate window KEY RESOURCES TABLE
Techniques: Recombinant, Hybridization, Membrane, Electrophoresis, Plasmid Preparation, Extraction, Purification, DNA Purification, Random Primed, DNA Labeling, Sequencing, Software
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